The kit “MutaGEL® Oxtress I” allows to detect the genotypes T-786C (promotor region) of the endothelial NOSynthase gene (NOS3) and C242T (His72Tyr) of the NAD(P)H Oxidase subunit CYBA in human genomic DNA probes.
Within the metabolic status named “oxidative stress” the production of molecules with free reactive electrones from e.g. glycolysis is not equalled by its normal potent lipidic or enzymatic electron acceptors. This causes diabetes, cancer and aging. The levels of several indicative molecules vary under the influence of some common DNA polymorphisms of enzymes, which metabolize the agonists of the radicalic or nonradicalic oxidants – mainly superoxide and hydroxide anion – such as T-786C of the NOSynthase promotor, C242T (His72Tyr) in a subunit of the NAD(P)H oxidase macromolecule. Additionally, the common complete deletion of glutathion-S-transferase genes M1 and T1 (see “MutaGEL® GST-M1/T1”) as well as sequence variation of superoxide-dismutase SOD2 and catalase (see “MutaGEL® Oxstress II”) influence the radicalic situation in the body.